Lapin anti-Boeuf (Vache) IgG (Heavy & Light Chain) Anticorps (FITC)

N° du produit ABIN965016

N° du produit (Fournisseur): 701-402-002

Aperçu rapide pour Lapin anti-Boeuf (Vache) IgG (Heavy & Light Chain) Anticorps (FITC) (ABIN965016)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Boeuf (Vache)

Hôte: Lapin

N° du produit (Fournisseur): 701-402-002

Fournisseur: Rockland

Fonction: F(ab')2 Bovine IgG (H&L) Antibody Fluorescein Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG and Bovine Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).  Limited reactivity will occur against Armenian Hamster IgG.

Attributs du produit: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogène: Optional[Immunogen]: Bovine IgG whole molecule

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Bovine IgG Fluorescein conjugated antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.